The Cult of Aku is an all-female cult that worships Aku, conducting ceremonies and wearing female outfits resembling him. Their leader, the High Priestess, gave birth to female septuplets, who she and the cultists raised to be elite assassins known as the Daughters of Aku, created solely to destroy Jack.
All of the members wear black cloaks with identical masks concealing their faces. They also wear headdresses that resemble Aku's horns.
Not much is known about how the cult was started, but they seem to hate Jack as much as Aku does. Towards the end of their training, the Daughters of Aku were made to kill most of the cult as a test of their skills.
- The cult is a strange phenomenon in the Samurai Jack's world since everyone in the world and the universe fear, hate and despise Aku due to his massive cruelty, brutality and tyranny. It is unknown why the Cult worships a demonic entity like Aku. As Aku seemingly entered into a depressed semi-reclusion some time in the last 50 years and is unaware of the cult, it seems that his absence might have inspired reactionaries.
- Contrary to the name of her cult, the High Priestess's dialog indicates that Aku has never personally interacted with her or anyone else related to their cult. It is implied that Aku himself does not seem to know, or remember, the existence of this cult dedicated to him.